ROB - Automated
96/384
qPCR set up

Part No: 65-2001 - ROB Single Channel Liquid Handling

System

• System include: cables, software, user manual and cooling blocks (one 96-well block, one 32 x 1.5 mL block, one mastermix and reagent loading block for 2 x 5 mL vials, 16 x 0,2 ml tubes, 16 x 1,5 ml tubes).
  

Speed Rob™ has two modes for doing qPCR set-up.

• Traditional mode uses one tip for every sample replicate and OneDip™ mode uses the same tip for all replicates doing serial dispensing.
• The example below is based on a qPCR set-up with three replicates and pre-made master mix.
  • One tip per replicate 96-plate: 27 minutes (18 µl mix + 2 µl sample) 384-plate: 95 minutes (5 µl mix + 2 µl sample)
  • OneDip™ 96-plate: 13 minutes (18 µl mix + 2 µl sample) 384-plate: 45 minutes (5 µl mix + 2 µl sample)
• Rob™ handles a 384 set-up with acceptable speed but with low investment cost.
• Software features qPCR set-up guide is a stepwise quide to design the protocol of qPCR experiment, optimize the use of reagents, several Master Mixes
• Serial dilution maximizes the accuracy of pipetting for serial dilution Sample normalization simply input two information, sample volume and concentration

• Accuracy and precision data obtained under optimized conditions may not be possible to reproduce in your application.
  
• The data was obtained under normal QPCR set-up conditions.
  
• On the other hand, high precision data indicates that the liquid handling is stable.
  
• Market leading precision can easily be reproduced at your lab. High precision and accuracy also grants that dispense performance is very robust.
  
• Changes in viscosity will not have the same impact on dispensed volume seen in competing systems. So the dispense data below is what you can expect from Rob™ in reality.

Liquid Level Detection (LLD) It is essential to have in-process LLD and tip detection to verify processed protocols.

• There are two methods used to measure the liquid level heights. Either conductive or pressure based systems are used.
  
• The advantages of pressure based LLD are that all liquids can be detected and the system isn’t sensitive for static electricity.
  
• Conductive measurements require expensive conductive tips, is sensitive to static electricity but measures faster.
  
• Rob™ uses a further developed pressure based LLD that has improved detection speed.
  
• The LLD system provides with better precision and sensitivity than needed.
  
• Detection limit: Detects as low 3 µl in a 200 µl PCR tube. Detection precision: ± 0.3 µl in a PCR tube with 20 µl volume.

• qPCR set-up guide: this will guide you to design the protocol of your qPCR experiment step-by-step.
  
• It optimizes the use of reagents, samples , controls and standards.
  
• The flexibility to use several Master Mixes in one run is also an advantage.
  
• Parameters in the protocol can be saved and modified easily, anytime.
  
• Serial dilution: compare to manual pipetting, automation will maximize accuracy.
  
• Information that you need to enter are dilution ratio, number of dilution steps, and position of standard and diluent.
  
• Sample normalization: you can normalize sample concentration with RobTM.