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ROB - Automated
96/384
qPCR set up


more

ROB Single channel liquid handling system





ROB



Intelligent and unique. Incredibly precise and easy to use. Rob™ offers smart software and exclusive OneDip™ technology. There is no extensive training required. Within 5 minutes, anyone can operate the software and create protocols. This is supported with accessible help menu in every window.






Part No: 65-2001 - ROB Single Channel Liquid Handling

System


  • System include: cables, software, user manual and cooling blocks (one 96-well block, one 32 x 1.5 mL block, one mastermix and reagent loading block for 2 x 5 mL vials, 16 x 0,2 ml tubes, 16 x 1,5 ml tubes).


Rob - speed





Speed Rob™ has two modes for doing qPCR set-up.


  • Traditional mode uses one tip for every sample replicate and OneDip™ mode uses the same tip for all replicates doing serial dispensing.
  • The example below is based on a qPCR set-up with three replicates and pre-made master mix.
    • One tip per replicate 96-plate: 27 minutes (18 µl mix + 2 µl sample) 384-plate: 95 minutes (5 µl mix + 2 µl sample)
    • OneDip™ 96-plate: 13 minutes (18 µl mix + 2 µl sample) 384-plate: 45 minutes (5 µl mix + 2 µl sample)
  • Rob™ handles a 384 set-up with acceptable speed but with low investment cost.
  • Software features qPCR set-up guide is a stepwise quide to design the protocol of qPCR experiment, optimize the use of reagents, several Master Mixes
  • Serial dilution maximizes the accuracy of pipetting for serial dilution Sample normalization simply input two information, sample volume and concentration


ROB - Accuracy & Precision





  • Accuracy and precision data obtained under optimized conditions may not be possible to reproduce in your application.
  • The data was obtained under normal QPCR set-up conditions.
  • On the other hand, high precision data indicates that the liquid handling is stable.
  • Market leading precision can easily be reproduced at your lab. High precision and accuracy also grants that dispense performance is very robust.
  • Changes in viscosity will not have the same impact on dispensed volume seen in competing systems. So the dispense data below is what you can expect from Rob™ in reality.




Liquid Level Detection (LLD )





Liquid Level Detection (LLD) It is essential to have in-process LLD and tip detection to verify processed protocols.


  • There are two methods used to measure the liquid level heights. Either conductive or pressure based systems are used.
  • The advantages of pressure based LLD are that all liquids can be detected and the system isn’t sensitive for static electricity.
  • Conductive measurements require expensive conductive tips, is sensitive to static electricity but measures faster.
  • Rob™ uses a further developed pressure based LLD that has improved detection speed.
  • The LLD system provides with better precision and sensitivity than needed.
  • Detection limit: Detects as low 3 µl in a 200 µl PCR tube. Detection precision: ± 0.3 µl in a PCR tube with 20 µl volume.


ROB - Software features





  • qPCR set-up guide: this will guide you to design the protocol of your qPCR experiment step-by-step.
  • It optimizes the use of reagents, samples , controls and standards.
  • The flexibility to use several Master Mixes in one run is also an advantage.
  • Parameters in the protocol can be saved and modified easily, anytime.
  • Serial dilution: compare to manual pipetting, automation will maximize accuracy.
  • Information that you need to enter are dilution ratio, number of dilution steps, and position of standard and diluent.
  • Sample normalization: you can normalize sample concentration with RobTM.


NW03-01, Cova Square,
Selangor Science Park 1,
Jalan Teknologi,
47810 Petaling Jaya,
Selangor, Malaysia
Tel: +603-6150 4612
Fax: +603-6142 4868
E . : info@biospensa.com





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