The U.S. Depart­ment of Energy looked at the space efficiency of a 2” cryo box and concluded the following, “For samples under 0.5ml, a cryo box 2” tall full of 2.0ml vials may be enormously wasteful.

Consider this arithmetic: A standard 2” cryo box has this total volume: 5 x 13.25 x 13.25 cm = 860ml. With 100 cells per box, each cell has a volume of 8.6ml, therefore a 1.0ml sample is only 12% space efficient, and a 0.2ml sample is only 3% space efficient.”